The FM820T is an upright fluorescence microscope with transmitted and reflected lighting, and a six-filter turret. The microscope is built around an infinity-corrected optical system with a 40X-1000X magnification range, and Koehler diascopic and episcopic illumination. The powerful 100W, wide-spectrum, mercury-vapor episcopic illuminator uses a rotary filter-selector which can house up to six fluorescence filter-blocks; already included are ultraviolet, violet, blue, and green excitation blocks. The sub-stage illuminator uses a 30W halogen lamp with field-lens and diaphragm for even, high-contrast lighting.
The backwards, quintuple nosepiece holds five plan-achromatic objective lenses, including: 4X, 10X, 20X, 40X, and 100X (oil). The Siedentopf-design head features a 30° incline, and a 48-75mm interpupillary-adjustment range. A trinocular photo-port provides a stable, dedicated mount for an imaging system. The microscope uses coaxial coarse and fine focus-adjustment, as well as coaxial control over X and Y translation of the 7.2″ x 5.6″ stage.
The 12MP color CCD digital camera and software suite provide a high-resolution imaging solution for fluorescence microscopy. The 1″ format CCD sensor balances resolution and sensitivity with its 3.1µm pixels, favoring higher resolution while retaining moderate sensitivity. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it’s a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.
Fluorescence
Filters |
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Filter Set |
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Chart |
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Excitation Filter |
Dichromatic Mirror |
Emission Filter |
Common Stains* |
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UV-1A |
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330-385nm |
400nm |
420nm |
DAPI, Aniline Blue, BFP, BAO 9, DANSA, Hoechst (33258, 33342, 34580) |
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V |
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400-410nm |
455nm |
455nm |
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B |
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460-495nm |
505nm |
510-550nm |
Acridine Orange (DNA), Acridine Yellow, Alexa Fluor (430, 488, 500, 514),Cy2, DiO, FITC, Lucifer Yellow |
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G2 |
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510-550nm |
570nm |
590nm |
Acridine Red, Alexa Fluor 532, NeuroTrace 530/615, Rhodamine B |
*Common stains are provided as examples, and vary in emission strength. Stain specifications should be verified against filter and light-source specifications. |
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Mercury HBO Excitation |
Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. Some fluorophores may emit weak signals despite falling within filter specifications.
Band |
Peak Wavelength |
UV |
334nm, 365nm |
V |
405nm |
B |
436nm |
G |
546nm |
Y |
578nm |
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Specifications
Optical System |
Infinity-corrected |
Head |
Trinocular, 30 ° incline, 360 ° rotatable |
Interpupillary Adjustment |
Compensation-free gemel, 48-75mm |
Eyepieces |
Plan 10X, high-eyepoint, FN 22mm, 30mm mount |
Photo-port |
23mm, compatible with reduction lenses |
Nosepiece |
Quintuple reversed |
Objective Lenses |
4X, 10X, 20X, 40X (spring), 100X (spring, oil) |
Focusing System |
Coaxial coarse and fine focus, upper limit-stop, tension-control |
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Focus Travel |
30mm, 0.002mm fine-adjustment |
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Stage |
Double-layer mechanical 185mm x 142mm |
X-Y Translation Range |
75mm x 55mm |
Sub-stage Condenser-holder |
Rack-and-pinion, centerable |
Condenser |
Achromatic swing condenser, NA0.9/0.25 |
Transmitted Illumination |
30W halogen, continuous adjustment |
Fluorescence Excitation Illumination |
100W mercury-vapor HBO arc-lamp |
Objective Lenses
Magnification |
Corrections |
NA |
Working Distance |
Cover-glass Thickness |
4X |
Plan achromatic |
0.10 |
28mm |
0.17mm |
10X |
Plan achromatic |
0.25 |
10mm |
0.17mm |
20X |
Plan achromatic |
0.40 |
5.1mm |
0.17mm |
40X |
Plan achromatic |
0.65 |
0.7mm |
0.17mm |
100X |
Plan achromatic |
1.25 |
0.18mm |
0.17mm |
Camera Specifications
Specifications
Sensor Specifications |
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Model |
Sony ICX834AQG (color) |
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Sensor Type |
interline CCD |
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Sensor Optical Format |
1″ |
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Active Pixels |
12.1M (4284 x 2836) |
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Pixel Size |
3.1µm x 3.1µm |
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Active Sensor Area |
13.28mm x 8.79mm |
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Shutter |
global shutter |
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Sensitivity |
420mV @ 1/30s (f/5.6) |
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Spectral Response |
380-650nm with IR-cut filter |
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Other Camera Specifications |
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Binning |
1×1, 2×2 |
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Video Capture Framerate |
3.6fps @ 4284×28363.6fps @ 2124×1418 |
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Photo Capture Resolution |
4284×28362124×1418 |
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Connectivity |
USB 3.0 |
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Lens-mount |
C-mount |
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Compensating Lens |
1X (optional) |
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Power |
5VDC over USB 3.0 |
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Dimensions |
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Operating Temperature |
-10-50°C |
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Operating Humidity |
30-80% |
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Software
OS Requirements |
Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+ |
Hardware Requirements |
Intel Core2 2.8GHz or comparable processor, 4GB RAM |
Features |
Windows Version |
Live video preview |
Captures still images and video |
Time-lapse capture |
Exposure and white-balance controls |
Dark-field correction |
Digital binning |
Image processing |
Measurement tools, including lines, arcs, polygons |
Segmentation and counting tools |
Image-stitching, extended depth-of-field, HDR |
Fluorescence color-mapping |
Mac/Linux Versions |
Live video preview |
Captures still images and video |
Exposure control |
Color adjustment |
Measurement tools, including lines, arcs, polygons |
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